The molecular bases of δ/αβ T cell-mediated antigen recognition

DG Pellicci; AP Uldrich; J Le Nours; F Ross; E Chabrol; SBG Eckle; R de Boer; RT Lim; K McPherson; G Besra; AR Howell; L Moretta; J McCluskey; MHM Heemskerk; S Gras; J Rossjohn; DI Godfrey

Journal article

The molecular bases of δ/αβ T cell-mediated antigen recognition

DG Pellicci, AP Uldrich, J Le Nours, F Ross, E Chabrol, SBG Eckle, R de Boer, RT Lim, K McPherson, G Besra, AR Howell, L Moretta, J McCluskey, MHM Heemskerk, S Gras, J Rossjohn, DI Godfrey

Journal of Experimental Medicine | Published : 2014

DOI: 10.1084/jem.20141764

Download PDF

Abstract

αβ and γδ T cells are disparate T cell lineages that can respond to distinct antigens (Ags) via the use of the αβ and γδ T cell Ag receptors (TCRs), respectively. Here we characterize a population of human T cells, which we term δ/αβ T cells, expressing TCRs comprised of a TCR-δ variable gene (Vδ1) fused to joining α and constant α domains, paired with an array of TCR-β chains. We demonstrate that these cells, which represent ~50% of all Vδ1+ human T cells, can recognize peptide-and lipid-based Ags presented by human leukocyte antigen (HLA) and CD1d, respectively. Similar to type I natural killer T (NKT) cells, CD1dlipid Ag-reactive δ/αβ T cells recognized α-galactosylceramide (α-GalCer); ho..

View full abstract

University of Melbourne Researchers

Daniel Pellicci Author

Adam Uldrich Author

Sidonia Eckle Author

McCluskey James Author

Related Projects (6)

RESEARCH FELLOWSHIP

Professor Godfrey is an immunologist with a long standing history as a pioneer in the study of a specialised type of white blood cell, known..

IMMUNE REGULATION EFFECTOR FUNCTION AND THERAPY

We seek to understand how white blood cells detect and destroy disease, and how molecules of the immune system punch holes in diseased cells..

RECOGNITION OF LIPID ANTIGENS BY THE IMMUNE SYSTEM.

T cells are a critical component of the immune system, protecting against a range of pathogens by detecting and responding to foreign molecu..

Atypical T cell receptor recognition of monomorphic CD1 antigen-presenting molecule

T lymphocytes are white blood cells that respond to foreign molecules (antigens). Until recently, most known antigens were proteins. This pr..

AN ADVANCED FLOW CYTOMETRY FACILITY FOR THE PETER DOHERTY INSTITUTE

The establishment of a flow cytometry facility in the new Peter Doherty Institute for Infection and Immunity will enhance capacity to invest..

ARC CENTRE OF EXCELLENCE FOR ADVANCED MOLECULAR IMAGING

The Centre of Excellence in Advanced Molecular Imaging will innovatively integrate physics, chemistry and biology to unravel the complex mol..

Grants

Awarded by National Institute of General Medical Sciences

Funding Acknowledgements

This work was supported by the Australian Research Council (ARC; DP110104124, CE140100011, and LE110100106), the National Health and Medical Research Council of Australia (NHMRC; #1013667), the Cancer Council of Victoria (#1042866), and National Institutes of Health R01 grant (GM 087136). D.G. Pellicci is supported by an NHMRC Biomedical fellowship, A.P. Uldrich by an ARC Future Fellowship (FT140100278), S. Gras by an ARC Future Fellowship (FT120100416), D.I. Godfrey by an NHMRC Senior Principal Research Fellowship (#1020770), and J. Rossjohn by an NHMRC Australia Fellowship (AF50).